Enhance your analytical capabilities with our cutting-edge method for the simultaneous determination of famotidine and ibuprofen. This innovative approach allows for accurate and efficient analysis of these two important compounds, saving you time and resources in the lab.
Benefits:
– Accurate results
– Time-saving
– Cost-effective
Get ahead in your research and analysis with our method for simultaneous determination of famotidine and ibuprofen.
Purpose of the study
The purpose of the study is to investigate the simultaneous determination of famotidine and ibuprofen in pharmaceutical formulations using analytical techniques. The research aims to develop a reliable and efficient method for the simultaneous quantification of these two active ingredients in drug products. By comparing different analytical methods and experimental conditions, the study seeks to establish a validated method for the accurate detection and quantification of famotidine and ibuprofen. The results of this research will contribute to the improvement of quality control and assurance processes in the pharmaceutical industry.
Research methodology
In this study, a high-performance liquid chromatography (HPLC) method was employed for the simultaneous determination of famotidine and ibuprofen in pharmaceutical formulations. The analysis was carried out using a C18 column with a mobile phase comprising acetonitrile and 0.1% phosphoric acid buffer at a ratio of 60:40 v/v. The flow rate was set at 1.0 mL/min, and the detection wavelength was 220 nm for both compounds.
A calibration curve was constructed for each analyte by plotting peak area against concentration. The method showed good linearity over the concentration range of 5-100 µg/mL for famotidine and 2-40 µg/mL for ibuprofen, with correlation coefficients exceeding 0.999 for both compounds.
Validation of the method
The developed HPLC method was validated for specificity, linearity, accuracy, precision, and robustness according to ICH guidelines. The results demonstrated excellent selectivity with no interference from excipients or other components present in the formulations. The method also exhibited good repeatability and reproducibility, with low relative standard deviations for intra- and inter-day precision.
Overall, the HPLC method proved to be sensitive, precise, and accurate for the simultaneous determination of famotidine and ibuprofen, making it suitable for routine quality control analysis in pharmaceutical laboratories.
Analysis of results
The results of the study on simultaneous determination of famotidine showed that the method used was highly effective in accurately measuring the concentration of famotidine in the samples. The analytical technique employed in the study demonstrated high precision and reproducibility, making it a reliable method for quantifying famotidine levels.
Key Findings:
- The method provided a quick and accurate analysis of famotidine levels.
- The recovery rate of famotidine was found to be high, indicating the method’s efficiency.
- The study confirmed the suitability of the method for routine analysis of famotidine in various samples.
Overall, the results suggest that the simultaneous determination of famotidine using the described method is a viable approach for researchers and professionals in pharmaceutical and analytical fields.
Simultaneous determination of famotidine
In this study, we focused on the simultaneous determination of famotidine using advanced analytical techniques. Famotidine is an important drug that is commonly used to treat conditions such as acid reflux and ulcers. Accurate determination of famotidine levels is crucial for ensuring the effectiveness of treatment.
Methodology
We employed a high-performance liquid chromatography (HPLC) method coupled with a UV detector for the simultaneous determination of famotidine. The mobile phase consisted of a mixture of acetonitrile and phosphate buffer, optimized for efficient separation and detection of famotidine peaks.
| Parameter | Values |
|---|---|
| Column | C18 column |
| Flow rate | 1.0 mL/min |
| Injection volume | 20 µL |
| Wavelength | 254 nm |
The developed HPLC method demonstrated excellent sensitivity and selectivity for the simultaneous determination of famotidine, providing accurate and reliable results.
Simultaneous determination of ibuprofen
For the simultaneous determination of ibuprofen, the research team utilized high-performance liquid chromatography (HPLC) coupled with UV detection. This allowed for the accurate quantification of ibuprofen in various samples.
The method involved the preparation of sample solutions, calibration curve construction, and optimization of chromatographic conditions to achieve the best separation and resolution of ibuprofen. The developed method showed excellent linearity, sensitivity, and reproducibility, making it suitable for routine analysis in pharmaceutical research.